Survivin Inhibitscaspase Activation
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Fig. I. Survivin inhibits Baxand Faa-inducedapoptosis in 293 cells. A, 293 cells were transfectedwith pcDNA3myc (control), pcDNA3myc-Survivin,pcDNA3myc-XIAP. pcDNA3-Bax, pcDNA3myc-Survivin+ pcDNA3-Bax, or pcDNA3myc-XIAP+ pcDNA3-Bax plasmidDNAs. One day later,the percentageof apoptoticcells (DAPI-staining)was determined (mean ±SE for three determinations). B, the expression of the transfected proteins was evaluated by immunoblot assay using 50 @agflaneof total protein. C, apoptosis was induced by transfection of pCMVFas, and the percentage of dead cells was determined I day later (mean ±SE for three determinations).
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Survivin Inhibitscaspase Activation
Fig. I. Survivin inhibits Baxand Faa-inducedapoptosis in 293 cells. A, 293 cells were transfectedwith pcDNA3myc (control), pcDNA3myc-Survivin,pcDNA3myc-XIAP. pcDNA3-Bax, pcDNA3myc-Survivin+ pcDNA3-Bax, or pcDNA3myc-XIAP+ pcDNA3-Bax plasmidDNAs. One day later,the percentageof apoptoticcells (DAPI-staining)was determined (mean ±SE for three determinations). B, the expression of the transfected p...
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